Skip to main content

Ion Microscopy Imaging of Boron from p-Boronophenylalanine in Surgically Acquired Samples of Human Brain Tumor Tissue

  • Chapter
Frontiers in Neutron Capture Therapy

Abstract

Using ion microscopy1 we are able to quantitatively image physiologically relevant isotopes, including 10B, in cancerous and contiguous normal tissues with cellular to subcellular resolution and with detection limits in the low-ppm to ppb range. Over the past several years we have been using this technique to analyze tissue sections from animal brain tumor models,13 as well as specimens prepared from tissue cultures,4,5 to develop appropriate cryogenic sampling procedures to be applied to the in situ analyses of 10B in human brain tumor specimens. An ion microscopy imaging study is presented which demonstrates the localization of 10B from the fructose complex of p-boronophenylalanine (BPA-F) in tumor cells from a patient with glioblastoma multi-forme. Patient A was infused intravenously for one hour to deliver 170mg BPA/kg body weight. The craniotomy commenced one-half hour after the end of the infusion. Tissue specimens from patient A were frozen on dry ice, stored at -80 °C, and at a later date cryosectioned and freeze-dried for ion microscopy analysis. The boron concentration in tumor cells, calculated from the grayscale intensity of ion images, was found to have a dynamic distribution with an average approximately 3.5 times higher than the 10B levels in the normal brain and blood. Ion images are shown to illustrate the complexities of cryogenic sample preparation for the analysis of 10B in human brain tumor tissues at the microscopic level.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. D.R. Smith, S. Chandra, J.A. Coderre, and G.H. Morrison, Ion microscopy imaging of 10B from p-boronophenylalanine in a brain tumor model for boron neutron capture therapy, Cancer Res., 56:4302–4306, 1996.

    PubMed  CAS  Google Scholar 

  2. D.R. Smith, S. Chandra, J.A. Coderre, R.F. Barth, W. Yang, L. Liu, J.L. Wilson, P.L. Micca, M.M. Nawrocky, J.H. Rotaru, and G.H. Morrison, Quantitative ion microscopy imaging of boron-10 in rat brain tumor models for BNCT, in: “Advances in Neutron Capture Therapy, Volume II, Chemistry and Biology”, B. Larsson, J. Crawford, and R. Weinreich, eds., Elsevier, New York, 1997, pp. 308–314.

    Google Scholar 

  3. D.R. Smith, D.D. Joel, J.A. Coderre, G.M. Morris, S. Chandra, M.M. Nawrocky, P.L. Micca, H. Patel, and G.H. Morrison, Ion microscopy imaging of boron in animal tissue sections for neutron capture therapy, in: “Frontiers in Neutron Capture Therapy,” M.F. Hawthorne, K. Shelly, R.W. Wiersema, eds., Kluwer Academic/Plenum Publishers, New York, 2001, pp. 893–897.

    Chapter  Google Scholar 

  4. S. Chandra, D.R. Lorey II, S.L. Lessig, D.R. Smith, and G.H. Morrison, Quantitative imaging of boron from BPA and BSH in glioblastoma cells with ion microscopy, in: “Advances in Neutron Capture Therapy, Volume II, Chemistry and Biology”, B. Larrson, J. Crawford, and R. Weinreich, eds., Elsevier, New York, 1997, pp. 315–320.

    Google Scholar 

  5. S. Chandra, D.R. Lorey, D.R. Smith, and G.H. Morrison, In vitro exposure of human T98G glioblastoma cells to mixed bnct drugs: Acquiring independent quantitative images of boron atoms delivered by 10BPA-F and 11BSH in the same cell by sims ion microscopy, in: “Frontiers in Neutron Capture Therapy,” M.F. Hawthorne, K. Shelly, R.W. Wiersema, eds., Kluwer Academic/Plenum Publishers, New York, 2001, pp. 905–909.

    Chapter  Google Scholar 

  6. S. Chandra, and G.H. Morrison, Ion microscopy in biology and medicine, Meth. Enzymol., 158:157–179, 1988.

    Article  PubMed  CAS  Google Scholar 

  7. W.A. Ausserer, Y.-C. Ling, S. Chandra, and G.H. Morrison, Quantitative imaging of boron, calcium, magnesium, potassium, and sodium distributions in cultured cells with ion microscopy, Anal. Chem., 61:2690–2695, 1989.

    Article  PubMed  CAS  Google Scholar 

  8. A.W. Robards, and U.B. Sleytr. “Low Temperature Methods in Biological Electron Microscopy,” Elsevier, New York, 1985, p. 67.

    Google Scholar 

  9. G.A. Rosenberg. “Brain Fluids and Metabolism,” Oxford University Press, New York, 1990, p. 179.

    Google Scholar 

  10. R.B. Martin, in: “Metal Ions in Biological Systems, Compendium on Magnesium and Its Role in Biology, Nutrition, and Physiology, Volume 26,” H. Sigel, and A. Sigel, eds., Marcel Dekker, Inc., New York, 1990, pp. 1–13.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2001 Springer Science+Business Media New York

About this chapter

Cite this chapter

Smith, D.R. et al. (2001). Ion Microscopy Imaging of Boron from p-Boronophenylalanine in Surgically Acquired Samples of Human Brain Tumor Tissue. In: Hawthorne, M.F., Shelly, K., Wiersema, R.J. (eds) Frontiers in Neutron Capture Therapy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1285-1_135

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-1285-1_135

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5478-9

  • Online ISBN: 978-1-4615-1285-1

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics